Population structure and variation in Persian sturgeon (Acipenser percicus ) from the Caspian Sea as determind from mitochondrial DNA sequences of the control region
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Abstract:
Mitochondria1 DNA (mtDNA) control region sequences were analyzed to evaluate the population genetic structure of Persian sturgeon (Acipenser persicus) in Caspian Sea. A total of 45 specimens were collected from the different locations of the Caspian Sea. MtDNA control region was amplified using PCR. Direct sequencing was performed according standard method. The results showed that 12 haplotypes were observed between 45 samples in the method. The highest numbers of haplotypes were observed in Sefidroud River in which 3 haplotypes A, B and E among them were specific for the river and were not observed in the other locations. The average haplotype diversity (h) and nucleotide diversity (π) were 0.795±0.037 and 0.0062±0.0046, respectively. The results of FST based on kimura- 2 parameters method and analysis of molecular variance (AMOVA) demonstrated that most variations occurred between samples from Sefidroud River in the south Caspian Sea and that the samples include three distinct populations including Sefidrud, Russia and Azerbaijan (P
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population structure and variation in persian sturgeon (acipenser percicus ) from the caspian sea as determind from mitochondrial dna sequences of the control region
mitochondria1 dna (mtdna) control region sequences were analyzed to evaluate the population genetic structure of persian sturgeon (acipenser persicus) in caspian sea. a total of 45 specimens were collected from the different locations of the caspian sea. mtdna control region was amplified using pcr. direct sequencing was performed according standard method. the results showed that 12 haplotypes...
full textGenetic diversity in the Persian sturgeon, Acipenser percicus, from the south Caspian Sea based on mitochondrial DNA sequences of the control region
The Persian sturgeon, Acipenser persicus (Borodin, 1897), is an economically important species, which mainly inhabits the Caspian Sea. However, little is known about its population genetic structure. In this study, variation in nucleotide sequences of the mitochondrial DNA (mtDNA) control region of wild stock Persian sturgeon was determined to assess the genetic diversity among different natura...
full textgenetic diversity in the persian sturgeon, acipenser percicus, from the south caspian sea based on mitochondrial dna sequences of the control region
the persian sturgeon, acipenser persicus (borodin, 1897), is an economically important species, which mainly inhabits the caspian sea. however, little is known about its population genetic structure. in this study, variation in nucleotide sequences of the mitochondrial dna (mtdna) control region of wild stock persian sturgeon was determined to assess the genetic diversity among different natura...
full textGenetic relationships among collections of the Persian sturgeon, Acipenser percicus, in the south Caspian Sea detected by mitochondrial DNA Restriction fragment length polymorphisms
In the present study, mitochondrial DNA polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was used to assess the population structure and genetic relationships among six Persian sturgeon, Acipenser persicus populations from south Caspian Sea along the Iranian coast. The complete nucleotide dehydrogenase subunit 5 (NADH 5) region of mtDNA amplified by PCR was di...
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Intraspecific sequence variation in the D-loop region of mtDNA in white sturgeon (Acipenser transmontanus), a relict North American fish species, was examined in 27 individuals from populations of the Columbia and Fraser rivers. Thirty-three varied nucleotide positions were present in a 462-nucleotide D-loop sequence, amplified using the polymerase chain reaction. Bootstrapped neighbor-joining ...
full textDevelopment of disomic single-locus DNA microsatellite markers for Persian sturgeon (Acipenser persicus) from the Caspian Sea
Understanding the scale at which wild stocks of Persian sturgeon (Acipenser persicus) are genetically discrete is necessary for effective management of this commercially important species. Disomic DNA microsatellite markers are among the best tools for determining stock structure in fishes. As all sturgeon species have a polyploid ancestry of all sturgeons, most gene loci exhibit more than two ...
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Journal title
volume 3 issue 2
pages 67- 80
publication date 2013-12-01
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